Question 1

What is Regenerative Organic Certified®?

Accepted Answer

This certification ensures farms and products meet the highest standards in history for soil health, animal welfare and farmer fairness–helping transform our health and the health of our planet for generations to come.

Question 2

Do I need to refrigerate these probiotics?

Accepted Answer

No. This formula includes probiotic strains that are designed to survive and thrive in the high temperatures and harsh environment of the gut. They are uniquely resilient and do not require refrigeration.

Question 3

Are all of the ingredients grown on Ancient Nutrition farms?

Accepted Answer

Our formulas are Regenerative Organic Certified®. To obtain that certification, they must contain 95% Regenerative Organic Certified® active ingredients grown on Regenerative Organic Certified® farms. The ingredients are grown on Regenerative Organic Certified farms, including ingredients grown on our founder-owned Regenerative Organic Certified® farms. That number excludes the capsule and probiotic cultures.

Question 4

What is Bacillus subtilis AB22?

Accepted Answer

Bacillus subtilis AB22 is a highly effective, live culture strain that was primarily developed by Ancient Nutrition founder and CEO Jordan Rubin. Ancient Nutrition is the first to use it. AB22 stands for Amplified Biotic and the 22 stands for the year it was introduced (2022). Known as a spore-forming SBO, Bacillus subtilis AB22 has all the benefits and qualities of Bacillus subtilis but functions at a higher level. Why is it important? -It can thrive in a hostile environment, like stomach acid - It acts as an “enzyme factory,” producing plant-digesting enzymes helping to produce short chain fatty acids Short chain fatty acids in general: - Help promote a healthy gut lining - Help create an inhospitable environment against undesirable microbes - Help supply energy to the human body

Question 5

What do the Regenerative Organic Certified® levels (bronze, silver and gold) mean?

Accepted Answer

There are three levels of Regenerative Organic Certified®: Bronze, Silver, and Gold. These levels apply to farms and producers only. Bronze represents the beginning level, and Gold represents the highest achievable level in regenerative organic production. This tiered approach enables farmers to adjust and adapt their practices over time and allows for continuous improvement. Because every brand that is Regenerated Organic Certified® needs to adhere to the strictest standards, we have to use the lowest certification label representative of the farm where we source our ingredients in the formula. However, our founder-owned farms in Missouri and TN are both certified Silver.

Question 6

Why did you combine soil-based organism probiotics (SBOs) with lactic acid bacteria probiotics (LABs)?

Accepted Answer

Soil-based organisms are often stronger than other forms of bacteria since they don't require refrigeration (meaning they're shelf-stable) or any special handling. They can generally survive the journey through the stomach to the intestines. Lactic acid bacteria (LABs) are typically refrigerated probiotics. They are usually freeze-dried and in a dormant or vegetative state. As soon as they reach moisture, these bacteria come out of dormancy and start to go to work, which also usually means they begin to die off. Soil-based organism probiotics (SBOs) have a natural shield. When they become activated by moisture, temperature or food, they stay in a spore state until they get to the gut versus beginning to die. LABs don’t have that luxury. Because SBOs arrive dormant and are stable at room temperature, without refrigeration, the LABs are shielded by the SBOs and can make their way to the gut. When SBOs start to work in the body, they create by-products that allow LABs to thrive. Plus, the noted potency is based on the SBOs. That means the colony-forming unit (CFU) count isn’t impacted when unrefrigerated and maintained throughout expiration. However, you should always read and follow label directions, for proper usage and how to store the product.

Question 7

What are the differences between SBO Probiotics, SBO Once Daily Probiotics, Regenerative Organic Certified® Probiotics and SBO Probiotics Trinity?

Accepted Answer

SBO Probiotics and SBO Probiotics Once Daily All of our SBO Probiotics formulas contain strains of soil-based organisms or SBOs, which are incredibly resilient and effective probiotics. Both SBO Probiotics Once Daily and SBO Probiotics are designed to support digestive health†, plus provide additional benefits beyond the gut. The formulas include time-tested, carefully chosen botanicals that have been traditionally used to support everything from mental clarity to gut health.† Our SBO Probiotics products (including Gut Restore†, Ultimate, Men’s and Women’s) contain a higher amount of Saccharomyces boulardii than the Once Daily products. Saccharomyces boulardii is a beneficial, tropical species of yeast ingredient that works as a probiotic to help support overall gut health and function.† It’s one of the few yeasts that survives best at the human body temperature, which helps it survive and thrive in the microbiome. SBO Probiotics Ultimate also contains the highest colony-forming unit (CFU) count of all our probiotics products, at 50 billion CFUs* per serving. The SBO Probiotics Once Daily products each contain 25 billion CFUs*. Each product offers targeted support for specific areas in addition to digestive health: they focus on mental clarity†, vaginal health†, healthy immune system support†, men’s health and women’s health. Our SBO Probiotics also include the strain Bacillus clausii, which is not in the Regenerative Organic Certified® Probiotics line. SBO Probiotics also have more herbs and nutrients in their formulas. That’s because Regenerative Organic Certified® Probiotics only have a 5% allowance of non-Regenerative Organic Certified® material. We had to be very selective with our ingredients to meet the Regenerative Organic Certified® requirements, so we chose to focus on including powerful myceliated mushroom ingredients from our founder-owned farms. Regenerative Organic Certified® Probiotics Our Regenerative Organic Certified® Probiotics are the first-ever triple certified line of products that are: Non-GMO, Certified Organic and Regenerative Organic Certified®. This certification goes beyond organic status to demand the highest standards in history for soil, animals and people. The Regenerative Organic Certified® Probiotics contain 95% of ingredients (excludes capsule and probiotic cultures) grown under the guidance of our founders on their Regenerative Organic Certified® farms as well as ingredients from other Regenerative Organic Certified® farms. It also means that 95% of the ingredients are produced on one or more of the farms located in Tennessee or Missouri as well as other Regenerative Organic Certified® farms. The remaining 5% are inactive ingredients, like the ingredients for the capsules. In addition, every single Regenerative Organic Certified® Probiotics formula has beneficial mushroom mycelium ingredients. Mushrooms are typically a great way to ferment these ingredients and are a source of prebiotics and postbiotics. While our SBO Probiotics lines only include SBO probiotics, our ROC™ Probiotics formulas also include lactic acid bacteria (LAB) cultures. These formulas combine a variety of 10 diverse probiotics strains. Soil-based organisms are generally more survivable than live cultures, which is why there are 10 strains of probiotics, including a yeast that functions as a probiotic and LAB probiotics in our Regenerative Organic Certified® Probiotics as well as SBOs. Our SBO Probiotics have three strains and one beneficial yeast. Regenerative Organic Certified® Probiotics varieties feature anywhere between 25 billion and 50 billion CFU1 per serving. SBO Probiotics Trinity SBO Probiotics Trinity also contains almost entirely SBO probiotic strains. In addition, it contains Lactobacillus LB fermentate, a proprietary, clinically studied blend of postbiotics and parabiotics. Postbiotics are produced through the fermentation process carried out by beneficial probiotic bacteria, and recent research shows that postbiotics can be just as beneficial when it comes to supporting your gut. It offers 20 billion CFUs* probiotics and 20 billion parabiotics. SBO Probiotics Trinity also includes a Prebiotic Blend composed of an Ayurvedic superfood fruit trio called the triphala blend, which is an herbal combination used for thousands of years to support overall gut and digestive health. It consists of three Indian fruits: amaliki or amla berry (known for its cooling effect), bibhitaki (used to help maintain balance within the body) and haritaki (known for its heating nature). 1At Expiration Date under recommended storage conditions. *at time of manufacture

Question 8

Why aren’t all of your products Regenerative Organic Certified®?

Accepted Answer

Achieving the Regenerative Organic Certified® was a huge milestone, and one that we’re incredibly proud of. It’s the highest standard in history, and isn’t easy to achieve. As we grow our farms and ingredients, we want to use Regenerative Organic Certified® ingredients wherever we can, even in our non-Regenerative Organic Certified® products – and eventually, we hope to have as many Regenerative Organic Certified® products across as many key lines as possible.

Question 9

What's the best time to take probiotics?

Accepted Answer

To help support absorption and colonization in the gut, we recommend taking probiotic capsules (or gummies) on an empty stomach, either when you first wake up or right before bed (roughly 2–3 hours after your last meal). However, you should always read and follow label directions for proper use and storage.

Question 10

Why should I give my gut support?

Accepted Answer

Fact: Most Americans don't consume enough probiotics in their diet due to modern processed foods, soil depletion and more. I believe most people are in need of probiotic support. Here's why: Thanks to prescription antibiotics, high carbohydrate diets, fluoridated tap water and high levels of stress, the good bacteria in our guts are often diminished, and our bodies can't replace them at the rate required for optimal health. Many probiotics health benefits are gut- and digestion-related, so here are a few key reasons why gut health, in general, is so important to overall health: - Up to 80% of the immune system is housed in the gut. - Between 80% to 90% of the body's serotonin, often referred to as a feel good or happy chemical which acts as a neurotransmitter, is found in the gut. Serotonin is thought to have many effects on the body, including the areas of digestion, sleep, memory, behavior and more. - Every bodily cell, tissue and organ depend on proper nutrient absorption and assimilation of food through healthy digestion, and, generally speaking, probiotics can play a supporting role in that.

Question 11

When will I start to notice a difference?

Accepted Answer

Everyone is different, and will have a different experience when using the products. We suggest giving any new product a chance for 90 days. However, some people will experience digestive support soon after they start.

Question 12

What is the right amount of CFUs to be getting every day?

Accepted Answer

It varies. For most people, we recommend a CFU count anywhere between 25 to 100 billion.

Question 13

Are your products produced in a facility with allergens?

Accepted Answer

We now have a disclaimer that is on ALL of our products: "Produced on equipment that may also process peanuts, tree nuts, milk, soybean, wheat, sesame, shellfish, fish, egg." This is a voluntary disclaimer and our formulations have not changed. Our manufacturers are required to have Policies and Procedures in place when it comes to handling and processing allergens. These allergen programs are in place to prevent cross contamination after each run by proper cleaning procedures. Allergen swab testing is also done after running an allergen containing product. We test finished products for allergens prior to designating as “Free” of. We also follow cGMP (current good manufacturing practices) which helps maintain allergen segregation.

Regenerative Organic Certified® Once Daily Probiotics